Objective To analyzed the surface electromyography (sEMG) characteristics of high-level male speed climbers using Tomoa skip technique, and provide a theoretical basis for the determination of special strength training methods and means. Methods Ten male speed climbers at national first level or above were recruited, and their kinematics and sEMG signal data using Tomoa skip technique during climbing the official route were collected. Results For using Tomoa skip technique, the order of the main muscle contribution rate of high-level male speed climbers was biceps brachii, triceps brachii, flexor digitorum superficialis, latissimus dorsi, anterior tibial, vastus lateralis, gluteus maximus, medial head of gastrocnemius muscle. The sum of all muscles contribution rates of the left side, the contribution rate of biceps brachii and triceps brachii were significantly lower than that of the right side (P<0 05). The contribution rate of medial head of left gastrocnemius muscle was significantly higher than that of right side (P<0 05). The level of activation of the left biceps brachii was significantly lower than that of the right (P<0.05). The co-contraction indexes of left elbow joint and ankle joint, right elbow joint and ankle joint were 0.93 ± 0.21,1.33 ± 0.14,0.72 ± 0.10, 2.08 ± 0.59, respectively. The co-contraction level of left elbow joint and ankle joint was significantly higher than that of the right side (P<0.05). Conclusions High-level male speed climbers using Tomoa skip technique showed obvious sEMG characteristics, the contribution rate of upper limb muscles and latissimus dorsi were higher than that of lower limb. The activation mode of elbow joint was dominated by biceps brachii, and that of ankle joint was dominated by anterior tibial muscle. There exsited differences between left and right limbs in coordinated movement mode. The difference of contribution rate and activation level of upper limb between the left and right limbs was more than that of the lower limb.