Objective To establish a finite element model of cell perfusion culture combined with computational fluid dynamics (CFD) to study the effect of different perfusion rates on the movement of suspended cells.Methods Two-dimensional model of cell and porous scaffold was established using COMSOL Multiphsics and meshed. According to different perfusion velocities, it was divided into three groups, namely, U0=1.96e_^-4m/s, U1=1.17e_^-4m/s and U2=0.4e_^-4m/s. The Fluid-structure interaction module was used for calculation.Results The flow field distribution in the culture chamber is relatively uniform. In the same time, the ratio of cell suspension instillation speed is U0: U1: U2=5:3:1, and the ratio of cell displacement in the porous scaffold is S0: S1: S2=4.1:2.9:1. In the case of proportionality, the displacement of the cells in the culture medium also roughly follows the corresponding proportional changes, indicating that the increase in the dripping speed promotes the growth of the cells in the suspension into the scaffold. Increasing instillation speed will cause stress concentration in cells during movement. The stress and fluid shear force (FSS) of cells during movement are within the safe value range, and cell destruction will not occur.Conclusions This method has important reference value for cell perfusion culture and has important significance for the design of bioreactor and the construction of functional tissue in vitro.