Objective: The parameters of lower limb kinematics, dynamics and muscle strength between beginners and professionals were compared to provide basis for scientific Tai Chi and formulation exercise prescription. Methods: The data of Tai Chi were collected by BTS infrared motion capture test system, Kistler three-dimensional forceplate, the kinematics, dynamics and muscle force parameters were calculated with AnyBody 7.0 simulation system. Results: (1) The knee flexion and extension angle, X-axis force of independent step of the professional group were significantly greater than experimental group (P < 0.01). (2) The lower limb muscle strength of the professional group was greater than experimental group in the bow step, virtual step, parallel step, horse step, servant step and independent step. The muscle strength of hemimembranous muscle, semitendinosus muscle and biceps femoris of horse step were greater than experimental group (P < 0.05). The muscle strength of gluteus maximus, gluteus medius, gluteus minimus, tensor fascia lata, vastus lateralis and biceps femoris of servant step were greater than experimental group (P < 0.05). The muscle strength of vastus lateralis, vastus medialis, biceps femoris and tibialis anterior of independent step were greater than experimental group (P < 0.05). Conclusion: In Tai Chi practice, the knee joint is the main force producing joint, and the lower limb strength of professionals is significantly greater than that of beginners. Independent step have great influence on hip joint, while lunge and footstep have great influence on knee joint. Different levels of Tai Chi exercisers have different degrees of activation of lower limb muscles. Beginners should focus on the muscles affected by different movements, so as to promote the coordinated development of lower limb muscles and improve the ability of posture control in Tai Chi practice.