Abstract: Objective To establish a biomechanical model of skeletal muscle of hand grasping and carry out reverse dynamics simulation to obtain the maximum muscle force of each muscle involved in the process of hand grasping under different forces. Methods CT scanning was performed on a volunteer's hand to obtain the CT data of his hand. The data were imported into Mimics software for 3D reconstruction to obtain the bone models of each segment After adjusting the model coordinates, studio imported the software of anybody, and used the software of anybody to establish the kinematics model of the hand skeleton, and added the related muscles involved in the flexion of each finger to get the skeletal muscle model of the hand. The model is used to simulate the reverse dynamics of hand grasping. Results The maximum muscle force of each muscle in the whole movement process was obtained after the external force of 5N ~ 30N was applied to each distal phalanx. With the increase of force, the maximum muscle force of each muscle showed a linear trend. For example, the maximum muscle force of flexor pollicis longus increased from 18.49n to 110.93n; and when the external force was 5N, the flexor pollicis brevis, flexor pollicis longus, adductor pollicis and little finger were obtained The maximum muscle force of short flexor was 7.70n, 18.49n, 9.49n and 8.39n. The muscle force of superficial and deep flexors was much greater than that of other muscles in the process of finger movement, which played a major role in grasping the hand. Conclusion The computer model for simulation analysis can effectively complete the research which is difficult to be completed by experiment; the results of the maximum muscle force of the muscles involved in the hand grasping at different resistance, and the relationship between muscle force of main muscles and joint angle can provide guidance and reference for the evaluation of hand rehabilitation effect of stroke patients, and also provide certain theoretical basis for the manufacture of rehabilitation equipment.