Objective To design and develop the mural coronary artery circumferential stress in vitro simulation device, to achieve the loading of mural circumferential stress in vitro under different degrees coronary myocardial bridge oppression. Methods Using the myocardial bridge coronary artery hemodynamics in vitro simulation device to achieve the measurement experiment of the circumferential stress of the mural coronary artery, and based on the this experimental data to achieve the loading of mural circumferential stress in vitro under different degrees coronary myocardial bridge oppression. Results Through the measurement experiment of mural coronary artery circumferential stress found that the mural coronary artery in heart period of circumferential stress and the maximum average fluctuation will increase significantly with the increase of the degree of myocardial bridge oppression. The load experiment waveform coincided basically with the measurement experimental waveform. Conclusions The device can realize the circumferential stress loading of the mural coronary artery in vitro, which provided an in vitro simulation platform that is as close as possible to the in vivo environment to explore the influence of hemodynamic anomalies of the proximal mural coronary artery on the occurrence of atherosclerosis and plaque rupture.