Objective: The single leg support pose of yoga was selected to compare the biomechanical characteristics of lower limb joint angle, joint force and muscle force of professional and non-professional groups, so as to provide theoretical basis for scientific fitness of yoga beginners and exercise prescription determined by rehabilitation physicians.Methods: BTS infrared motion capture system was used to collect kinematic data, Kistler forceplate was used to collect dynamic data, Anybody 7.0 simulation software was used to calculate the joint force and muscle strength, Biodex balance system was used to evaluate the stability of single support. Results: (1) In both movements, the muscle force of lower limbs in the professional group was greater than the non-professional group. Support leg of Tree pose: The muscle strength of the tibialis anterior, gluteus medius posterior, iliac medialis and sartorius in the professional group was greater than the non-professional group (P<0.05). Balance leg: The muscle strength of the biceps femoris breve, sartorius, piriformis, gemellus inferior, iliac lateralis, iliac medialis, gluteus maximus inferior, obturator internus in the professional group was greater than the non-professional group (P<0.05). Support leg of Yan balabce: The muscle strength of tibialis anterior, extenso digitorum longus, extensor hallucis longus, obturator internus, gluteus maximus superior in the professional group was greater than the non-professional group (P<0.05). Balance leg: The muscle strength of extensor hallucis longus in the professional group was greater than the non-professional group(P<0.05). (2) The scores of professional group of front-rear, overall of PS-BI, overall, front-rear, left-right of ASL were significantly lower than the non-professional group(P<0.05). Conclusion: The size of joint angle affects the degree of muscle exercise of lower limbs. During the exercise, we should pay attention to the standard of movement and exercise the corresponding muscles. In the tree pose, the muscle strength of both legs in the non-professional group was significantly different in the professional group, and the difference in the Yan balance pose was more obvious on the supporting leg. Yoga exercises can improve the stability of one leg support in multiple directions.