Objective To analyze the effects of different sensory integration tasks on biomechanical characteristics of the lower limbs during walking in female patients with patellofemoral pain (PFP), and exlpore the relationship between these characteristics and patellofemoral joint stress (PFJS), so as to provide a reference for the prevention of PFP recurrence. Methods A total of 36 female patients with PFP were recruited for the study, and kinematic and kinetic data were collected while the subjects were walking. The effects of baseline, tactile integration task, listening integration task and visual integration task, on lower limb biomechanical characteristics was evaluated through the use of one-way ANOVA. Pearson correlation analysis was used to analyze the relationship between the above biomechanical characteristics and PFJS. Results Compared to the baseline, step length (P=0.004) and cadence (P=0.001) were significantly reduced in patients with PFP when performing the listening integration task. Furthermore, both variables exhibited a negative correlation with PFJS. Both the hip internal rotation angle (P=0.001) and the ankle internal rotation angle (P=0.022) were significantly increased in tactile integration task. Furthermore, the aforementioned metrics were significantly and positively correlated with PFJS (hip internal rotation angle: P<0.001; ankle internal rotation angle: P<0.001). The hip internal rotation angle (P=0.001), knee flexion angle (P=0.019), ankle inversion angle (P=0.003), and ankle plantarflexion angle (P=0.048) were increased in patients with PFP performing the visual integration task. These biomechanical characteristics exhibited a positive correlation with PFJS (P<0.050). Conclusions The listening integration task decreases step length and cadence in patients with PFP, and the tactile and visual integration tasks increase hip internal rotation angle and ankle internal rotation angle. On this basis, the visual integration task also increases the knee flexion angle and ankle plantarflexion angle during walking. Overall, it appears that the visual integration task has the greatest effect on gait biomechanics in female patients with PFP, and changes in these biomechanical characteristics are associated with elevated PFJS.