目的 探究沟槽拓扑基底形貌对宫颈癌 HeLa 细胞形态、迁移速率的影响。 方法 在 4 种不同表面特征(平面、槽宽 10 μm 平行沟槽、槽宽 20 μm 平行沟槽、分叉沟槽) PDMS 基底上培养 HeLa 细胞,采用免疫荧光技术对HeLa 细胞转染 F-actin,并用特异性探针标记线粒体,通过活细胞工作站获取细胞在不同时刻的位置、形态、线粒体分布。 结果 槽宽 10 μm 平行沟槽中 HeLa 细胞较槽宽 20 μm 平行沟槽和平面基底排列更加有序,形态更加细长,但平均迁移速率更低;分叉口处 HeLa 细胞向分叉结构中伸出突出,线粒体主要分布在突出处和细胞核周围,分叉口的存在降低了槽宽 10 μm 平行沟槽中 HeLa 细胞的平均迁移速率。 结论 沟槽拓扑基底形貌对 HeLa 细胞的形态和迁移速率有明显影响。 研究结果有助于了解体内微环境中拓扑结构在影响 HeLa 细胞迁移过程中的作用,并为后续关于宫颈癌侵袭与转移的研究提供参考。
Objective To investigate the effects of groove topography on morphology and migration speed of cervical cancer HeLa cells. Methods HeLa cells were cultured on PDMS substrates with four different surface features, namely, flat substrate, 10 μm width parallel groove, 20 μm width parallel groove, bifurcate groove. Immunofluorescence technique was used to transfect F-actin in HeLa cells, and specific probes Mito-Tracker Green were used to label mitochondria. The location, morphology of cells and distribution of mitochondrial at different moments were obtained through the living cell system. Results Compared with 20 μm width parallel groove and flat substrate, HeLa cells in 10 μm width parallel groove were more orderly arranged and more elongated, but their migration speed was much slower. HeLa cells at the bifurcation protruded into branches and mitochondria were mainly distributed at the protrusion and around the nucleus. The bifurcation reduced the average migration speed of HeLa cells in 10 μm width parallel groove. Conclusions Groove topography has a significant effect on morphology and migration speed of HeLa cells. The research findings help to understand the role of topography in in vivo microenvironment during migration of HeLa cells, and provide references for the subsequent research on invasion and metastasis of cervical cancers.
贾瑞洁,王 立,安美文.沟槽拓扑基底形貌对宫颈癌 HeLa 细胞迁移行为的影响[J].医用生物力学,2023,38(2):255-260复制