探究灌注培养中速度对悬浮细胞在多孔支架中运动的影响
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1.太原理工大学 生物医学工程学院;2.太原理工大学 机械与运载工程学院

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R 318.01

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To investigate the effect of velocity on the movement of suspension cells in porous scaffolds during perfusion culture
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    摘要:

    目的 结合计算流体动力学(computational fluid dynamics,CFD)建立细胞灌注培养有限元模型,研究不同灌注速度对悬浮细胞运动的影响。方法 利用COMSOL Multiphsics建立细胞和多孔支架二维模型并进行网格划分,根据灌注速度不同分为三组,分别为U0=1.96e-4m/s、U1=1.17e-4m/s、U2=0.4e-4m/s,利用流固耦合模块进行计算。结果 培养腔中流场分布是比较均匀的。相同时间内,细胞悬液滴注速度之比为U0:U1:U2=5:3:1,细胞在多孔支架中运动的位移之比为S0:S1:S2=4.1:2.9:1,在速度成比例的情况下,培养液中细胞的位移也大致遵循相应的比例变化,说明滴注速度的增大对悬液中的细胞向支架内部生长起促进作用。滴注速度增加会使细胞在运动过程中出现应力集中现象,细胞在运动过程中所受应力及流体剪切力(FSS)均在安全值范围内,不会出现细胞破坏现象。结论 该方法对细胞灌注培养有重要参考价值,对生物反应器的设计和体外功能组织的构建具有重要意义。

    Abstract:

    Objective To establish a finite element model of cell perfusion culture combined with computational fluid dynamics (CFD) to study the effect of different perfusion rates on the movement of suspended cells.Methods Two-dimensional model of cell and porous scaffold was established using COMSOL Multiphsics and meshed. According to different perfusion velocities, it was divided into three groups, namely, U0=1.96e-4m/s, U1=1.17e-4m/s and U2=0.4e-4m/s. The Fluid-structure interaction module was used for calculation.Results The flow field distribution in the culture chamber is relatively uniform. In the same time, the ratio of cell suspension instillation speed is U0: U1: U2=5:3:1, and the ratio of cell displacement in the porous scaffold is S0: S1: S2=4.1:2.9:1. In the case of proportionality, the displacement of the cells in the culture medium also roughly follows the corresponding proportional changes, indicating that the increase in the dripping speed promotes the growth of the cells in the suspension into the scaffold. Increasing instillation speed will cause stress concentration in cells during movement. The stress and fluid shear force (FSS) of cells during movement are within the safe value range, and cell destruction will not occur.Conclusions This method has important reference value for cell perfusion culture and has important significance for the design of bioreactor and the construction of functional tissue in vitro.

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  • 收稿日期:2020-12-16
  • 最后修改日期:2021-02-01
  • 录用日期:2021-02-03
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