负荷渐增式训练对老年小鼠骨骼肌卫星细胞AMPK磷酸化的影响
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重庆医科大学附属康复医院

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Effects of Incremental training on phosphorylation of AMPK in skeletal muscle satellite cells of aged mice
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The Affiliated Rehabilitation Hosptial of Chongqing Medical University

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    摘要:

    【摘要】目的 探讨负荷渐增式训练对老年小鼠骨骼肌卫星细胞腺苷酸活化蛋白激酶(AMP-activated protein kinase,AMPK)磷酸化的影响。方法 选取17月龄老年雄性C57BL/6小鼠24只,6周龄青年雄性C57BL/6小鼠12只,作为青年对照组(YC),将老年雄性小鼠随机分为老年对照组(OC)和老年运动组(OT),每组12只,单笼饲养。运动组小鼠进行6周负荷渐增式训练,对照组小鼠笼内正常生活,不运动,最后一次运动后24h取材,流式细胞分选技术分离CD45-/CD31-/Sca1-/VCAM(CD106)+细胞群体,分选细胞通过Myod肌原性染色以及成肌分化诱导培养进行卫星细胞鉴定,免疫组化结合Western blot法检测肌卫星细胞p-AMPK水平;结果 与老年对照组比较,青年对照组小鼠骨骼肌卫星细胞p-AMPK水平显著升高,老年运动组p-AMPK水平显著升高;结论 负荷渐增式训练可促进老年小鼠骨骼肌卫星细胞AMPK磷酸化,改善老年小鼠骨骼肌能量代谢。

    Abstract:

    [Abstract] Objective To investigate the effect of Incremental training on the adenylate-activated protein kinase (AMPK) phosphorylation of skeletal muscle satellite cells in aged mice. Methods A total of 24 17-month-old male C57BL/6 mice and 12 6-week-old young male C57BL/6 mice were selected. The aged mice were randomly divided into a control group and anIncremental training group, with 12 in each group, Cage rearing. The mice in the exercise group lasted for 6 weeks. The mice in the control group lived normally in the cages without exercise. Samples were taken 24 hours after the last exercise. CD45- / CD31- / Sca1- / VCAM (CD106) + cells were isolated by flow cytometry sorting. Myogenic molecules Myod was used to the sorted cells. The cells were also cultured and induced to differentiate. immunohistochemistry combined with Western blot to detect p-AMPK levels in muscle satellite cells; Results compared with the old control group, The level of p-AMPK in young control group satellite cells was significantly increased, and the level of p-AMPK in the agedIncremental training group was significantly increased. Conclusion Endurance xercise can increase the level of AMPK phosphorylation in skeletal muscle of aged mice and improve the energy metabolism of skeletal muscle in elderly mice.

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  • 收稿日期:2020-07-07
  • 最后修改日期:2020-09-08
  • 录用日期:2020-09-10
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