BPX-80离心式血泵中剪切应力引发血管性血友病因子机械损伤的评价研究
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苏州大学 机电工程学院

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国家自然科学基金青年(31700817),江苏省自然科学基金青年(BK20170352),江苏省高校自然科学研究面上项目(17KJB416010),第62批国家博士后基金(2017M620224)


Evaluation on mechanical damage of vWF induced by shear stress in BPX-80 centrifugal blood pump
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Artificial Organ Technology Laboratory, Bio-manufacturing Research Centre, School of Mechanical and Electrical Engineering, Soochow University

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    摘要:

    摘要:目的 对BPX-80离心式血泵中剪切应力对血管性血友病因子(von Willebrand Factor,vWF)的损伤进行评价,判断其是否可以作为vWF损伤研究的参照泵,从而对其他新型血泵在vWF损伤方面提供一些参考。方法 按照ASTM标准搭建体外溶血测试平台,使用新鲜猪血在此平台对BPX-80离心式血泵进行了8h的测试,并与静置对照组在溶血水平和血管性血友病因子(von Willebrand Factor,vWF)损伤方面进行对比评估。结果 在整个实验过程中BPX-80的溶血指数保持稳定且较低,前6h游离血红蛋白水平与静置对照组无显著性 差异;高分子量vWF多聚体有少量降解,与静置对照组相比无显著性差异;vWF抗原含量无明显变化与静置对照组趋势基本一致。结论 BPX-80离心式血泵血液相容性良好,可以作为vWF损伤以及溶血评价实验的基准参照泵,从而对新型血泵的设计与优化提供参考,以减少临床消化道出血并发症。

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    Abstract:Objective Evaluate the damage of von Willebrand Factor (vWF) induced by shear stress in BPX-80 centrifugal blood pump, and determine whether it can be used as a reference pump for vWF injury research, so as to provide some guidance for vWF damage evaluation. Method An in vitro hemolysis test platform was established according to the ASTM standards. The BPX-80 centrifugal blood pump was tested for 8 hours by using fresh porcine blood. The hemolysis level and von Willebrand Factor (vWF) damage of hourly blood samples were then evaluated and compared with the static control group. Results The hemolysis index of BPX-80 is stable and low during the 8-hour-test. The level of free hemoglobin in the first 6 hours is not significantly different from that of the static control group; the high molecular weight vWF polymer has a small amount of degradation, and shows no significant difference compared with the static control group; there is no significant change in the concentration of vWF antigen, which was basically consistent with the trend of the static control group. Conclusions It shows that the BPX-80 centrifugal blood pump has good blood compatibility and can be used as a reference pump for vWF damage and hemolysis evaluation, thereby providing guidance for the design and optimization of new blood pumps to reduce clinical gastrointestinal bleeding complications.

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  • 收稿日期:2020-07-02
  • 最后修改日期:2020-08-06
  • 录用日期:2020-08-06
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