可控压缩应力轴向诱导山羊椎间盘退变模型构建及评价
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1.重庆郭昌毕中医骨伤科医院;2.南华大学附属第一医院

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基金项目:

重庆市科卫联合中医药科研基金资助重点项目(No.2019ZY013438);


Construction and evaluation of a model of goat intervertebral disc degeneration induced by axial compressive stress
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Affiliation:

1.the first affiliated hospital of south China university;2.Chongqing fuling guo changbi orthopedic hospital

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key project supported by chongqing science and health research foundation of traditional Chinese medicine (No.2019ZY013438);

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    摘要:

    目的:探讨可控压缩应力轴向诱导山羊椎间盘退变模型构建,评价影像学及病理学的构建特点。方法:山羊20只,随机分4组(1对照组,2-4实验组,每组5只)。采用自制轴向弹性应力加压装置分别对2-4组轴向诱导(4周、8周、12周)山羊椎间盘施加40N轴向压缩应力,1组作对照组(不加压力)。DHI(DR)、Thompson分级法(MRI)观察椎间盘高度及退变程度,HE染色及Ⅰ、Ⅱ型胶原免疫组化染色在椎间盘组织学中的改变。结果:对照组:山羊椎间盘随着轴向加压诱导时间的延长椎间盘DHI、Pfirrmann分级、HE染色及免疫组织化学染色均无统计学意义(P>0.05)。实验组:DHI随时间延长而椎间盘高度减小有统计学意义(P<0.05),Pfirrmann分级随时间延长分级越高III→V;HE染色椎间盘髓核体积缩小、髓核细胞减少,逐渐被纤维样组织代替。免疫组织化学染色髓核I型胶原染色由淡黄色→黄色→棕黄色;髓核II型胶原染色由较深黄色→黄色→淡黄色;提示椎间盘I型胶原蛋白逐渐增多,II型胶原蛋白逐渐减少,椎间盘出现退变。结论:一定的轴向压缩应力诱导山羊椎间盘随着时间延长退变加重,轴向压缩应力诱导的时间直接影响椎间盘退变程度,构建山羊大动椎间盘退变物模型具有可重复性和可行性。

    Abstract:

    Abstract: objective: To explore the model of axial compression stress induced disc degeneration in goats and evaluate the imaging and pathological features. Methods: Methods: 20 goats were randomly divided into 4 groups (1 control group, 2-4 experimental group, 5 goats in each group).40N axial compressive stress was applied to goat intervertebral discs induced in 2 to 4 groups (4 weeks, 8· weeks, 12 weeks) by self-made axial elastic stress pressure device, and 1 group was used as the control group (no pressure was added).The height and degeneration of intervertebral disc were observed by DHI(DR) and Thompson grading (MRI). The histological changes of intervertebral disc were observed by HE staining and immunohistochemical staining of type 1 and type 11 collagen. Results: in the control group, DHI, Pfirrmann grading, HE staining and immunohistochemical staining of goat intervertebral disc were not statistically significant with the extension of axial compression time (P >0.5).In the experimental group, DHI decreased with the extension of time, which was statistically significant (P < 0.05).HE staining reduced the volume of nucleus pulposus and reduced the number of nucleus pulposus cells, which were gradually replaced by fibrous tissue.Immunohistochemical staining of nucleus pulposus type I collagen was changed from light yellow to yellow to brown-yellow.The staining of type II collagen in nucleus pulposus was from dark yellow to yellow to light yellow.The results indicated that collagen type I increased gradually and collagen type II decreased gradually, and degeneration occurred in the disc. Conclusion: certain axial compression stress can induce the degeneration of lumbar intervertebral disc in goats with the extension of time, and the time of axial compression directly affects the degeneration degree. Therefore, it is reproducible and feasible to construct the animal model of intervertebral disc degeneration.

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  • 收稿日期:2020-06-25
  • 最后修改日期:2020-09-05
  • 录用日期:2020-09-10
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