全身振动训练后骨质疏松大鼠股骨组织生物力学与Wnt3a蛋白表达的变化
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贵州师范学院体育学院

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贵州省体育局课题,课题编号:GZTY2018202. 贵州省科技计划项目(黔科合基础[2017]1035)


Changes of biomechanics and Wnt3a protein expression in femur of osteoporotic rats after whole body vibration training
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College of Physical Education, Guizhou Normal University

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    摘要:

    摘要:目的 探讨全身振动训练对股骨组织生物力学与Wnt3a蛋白表达的影响。方法 取雌性SD大鼠48只,随机分为假手术组、骨质疏松组与全身振动组,每组16只。骨质疏松组与全身振动组大鼠采用切除双侧卵巢方法复制骨质疏松模型,术后第11周,利用振动训练平台对全身振动组进行运动训练,振幅为2mm,振动频率30Hz,每次振动20min,每周干预5次,总共干预12周;假手术组与骨质疏松组大鼠在笼中自由活动。最后一次干预后的24h取右侧股骨,采用Micro-CT检测骨形态计量学参数,三点弯实验检测骨结构力学参数与材料力学参数,Western blotting检测Wnt3a、β-catenin蛋白表达量,qRT-PCR检测Wnt3a、β-catenin、cyclin D1、Tcf1基因表达量。结果 与假手术组相比较,骨质疏松组骨密度、骨体积分数、骨小梁数量、骨小梁厚度均降低(P<0.01),骨小梁间隙升高(P<0.01);与骨质疏松组比较,全身振动组骨密度、骨体积分数、骨小梁数量、骨小梁厚度均升高(P<0.05),骨小梁间隙降低(P<0.05)。与假手术组比较,骨质疏松组最大载荷、弹性载荷、最大挠度降低(P<0.05);与骨质疏松组比较,全身振动组最大载荷、弹性载荷、最大挠度升高(P<0.05)。与假手术组比较,骨质疏松组最大应力、弹性应力、最大应变和弹性模量降低(P<0.05);与骨质疏松组比较,全身振动组弹性应力、最大应变和弹性模量升高(P<0.05)。与假手术组比较,骨质疏松组Wnt3a、β-catenin蛋白及基因表达降低(P<0.05),cyclin D1、Tcf1基因表达量降低(P<0.05);与骨质疏松组比较,全身振动组Wnt3a、β-catenin蛋白及基因表达升高(P<0.05),cyclin D1、Tcf1基因表达量升高(P<0.05)。结论 全身振动训练可改善骨质疏松大鼠股骨生物力学性能,提高其Wnt3a蛋白表达。

    Abstract:

    Abstract:Objective To investigate the effect of whole body vibration training on biomechanics and Wnt3a protein expression of femur.Methods 48 female SD rats were randomly divided into sham operation group, osteoporosis group and whole body vibration group, 16 in each group.Rats in the osteoporosis group and the whole body vibration group were excised bilateral ovaries to duplicate the osteoporosis model. At the 11th week after operation, the vibration training platform was used to train the whole body vibration group with the amplitude of 2mm, the vibration frequency of 30Hz, each vibration for 20min, five times a week, a total of 12 weeks; rats in the sham operation group and the osteoporosis group were free to move in the cage.After the last intervention, the right femur was taken for 24 hours, the bone morphometric parameters were measured by micro CT, the mechanical parameters of bone structure and materials were measured by three-point bending test, the protein expression of Wnt3a and β - Catenin was measured by Western blotting, and the gene expression of Wnt3a, β - Catenin, cyclin D1 and tcf1 were detected by qRT-PCR.Results Compared with the sham operation group, the bone density, bone volume fraction, bone trabecular quantity and bone trabecular thickness of the osteoporosis group were significantly reduced (P < 0.01), and the bone trabecular space was significantly increased (P < 0.01); compared with the osteoporosis group, the bone density, bone volume fraction, bone trabecular quantity and bone trabecular thickness of the whole body vibration group were significantly increased (P < 0.05), and the bone trabecular space was significantly reduced (P < 0.05).Compared with the sham operation group, the maximum load, elastic load and deflection of the osteoporosis group were significantly reduced (P < 0.05); compared with the osteoporosis group, the maximum load, elastic load and deflection of the whole body vibration group were significantly increased (P < 0.05).Compared with the sham operation group, the maximum stress, elastic stress, maximum strain and elastic modulus in the osteoporosis group decreased significantly (P < 0.05); compared with the osteoporosis group, the elastic stress, maximum strain and elastic modulus in the whole body vibration group increased significantly (P < 0.05).Compared with the sham operation group, Wnt3a, β - Catenin protein and gene expression decreased (P < 0.05), cyclin D1, tcf1 gene expression decreased (P < 0.05); compared with the osteoporosis group, Wnt3a, β - Catenin protein and gene expression increased (P < 0.05), cyclin D1, tcf1 gene expression increased (P < 0.05).Conclusion Whole body vibration training can improve the biomechanical properties of femur and the expression of Wnt3a protein in osteoporotic rats.

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  • 收稿日期:2020-06-09
  • 最后修改日期:2020-07-09
  • 录用日期:2020-07-16
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