DNA吸附膜热膨胀系数对环境条件的依赖性
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1.上海大学;2.常州大学;3.上海大学,上海市应用数学和力学研究所,上海市力学在能源工程中的应用重点实验室

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国家自然科学基金项目(面上项目,重点项目,重大项目),上海市教委重大项目


The Dependency of Thermal Expansion Coefficient of DNA Adsorption Film on Environmental Conditions
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1.Shanghai University;2.Changzhou University;3.Shanghai University, Shanghai Key Laboratory of Mechanics in Energy Engineering, Shanghai Institute of Applied Mathematics and Mechanics

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The National Natural Science Foundation of China (General Program, Key Program, Major Research Plan), The Major Program of the Shanghai Municipal Education Commission

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    摘要:

    目的 研究基底上DNA吸附膜热膨胀系数对环境条件的依赖性。方法 将DNA吸附分子视为有预应变的宏观连续介质薄膜,建立了DNA膜?基底等效层合梁模型,预测温度载荷作用下DNA?微悬臂梁挠度;采用Parsegian经验势描述DNA吸附膜的介观自由能,利用DNA液晶?基底多尺度挠度模型、思想实验法和等效变形法,建立了DNA吸附膜微观结构与其宏观力学性能的跨尺度关系,预测了基底上DNA吸附膜的热膨胀系数。结果 在给定离子强度条件下,双链DNA吸附膜的热膨胀系数为0.3~8.05×10?4 K?1,单链DNA吸附膜的热膨胀系数为1.28~9.33×10?4 K?1。结论 在微观作用竞争中起主导作用的构型熵变化决定了DNA吸附膜热膨胀系数对环境条件的依赖性;随着温度或离子浓度或DNA种植密度的增加,DNA膜热膨胀系数减小;不同于体相溶液中DNA分子,基底上DNA吸附膜的热膨胀系数与温度变化弱相关,而与离子浓度和种植密度变化强相关,其中对种植密度变化最为敏感。这些结果对于基因检测及其调控具有指导意义,并为组织工程中组织器官的性能评估提供参考。

    Abstract:

    Object To investigate the dependency of thermal expansion coefficient of DNA adsorption film on environmental conditions. Methods By treating DNA adsorption film as a macroscopic continuum film with prestrain, an equivalent composite beam model of DNA film-substrate was established to calculate the deflection of DNA-microcantilever beam under temperature loading; With Parsegian’s empirical potential describing the mesoscopic free energy of DNA adsorption film, the DNA liquid crystal-substrate multiscale deflection model, the thought experiment method and the equivalent deformation method are combined to establish the trans-scale relationship between the microstructure of DNA adsorption film and its macroscale mechanical properties. Thereout the thermal expansion coefficient of DNA adsorption film was predicted. Results Given the ionic strength, the thermal expansion coefficient of double-stranded DNA adsorption film ranges from 0.3×10?4 K?1 to 8.05×10?4 K?1, and that of single-stranded DNA adsorption film ranges from 1.28×10?4 K?1 to 9.33×10?4 K?1. Conclusions As a leading role in the competition of micro-interactions, the change of configurational entropy determines the dependency of thermal expansion coefficient of DNA adsorption film on environmental conditions; the thermal expansion coefficient of DNA adsorption film decreases with the increase of temperature or ion concentration or DNA packing density; in contrast with DNA molecules in bulk solution, the thermal expansion coefficient of DNA adsorption film on substrate is weakly correlated with temperature change, whereas strongly correlated with ion concentration change and packing density change, among of which the packing density change is the most sensitive factor. These results are useful for gene detection and its regulation, and provide reference for the evaluation of tissue organ performance in tissue engineering.

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  • 收稿日期:2020-01-05
  • 最后修改日期:2020-03-13
  • 录用日期:2020-03-17
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