一种针刺补泻法对小型猪皮下组织液压的影响
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1.中国中医科学院针灸研究所;2.北京中医药大学针灸推拿学院

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国家自然科学基金资助项目(No.81173206) 通信作者:张维波,教授,博士生导师,Email:1507307638@qq.com *


Mechanism of the Hydraulic Pressure in Subcutaneous Connective Tissue on Mini Pigs Effected by a Reinforcing and Reducing Method of Acupuncture
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1.Institute of Acu-moxibustion,China Academy of Chinese Medical Sciences,Beijing;2.School of Acu-moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing;3.Institute of Acu-moxibustion,China Academy of Chinese Medical Sciences,Beijing,;4.China;5.School of Acu-moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing,

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    摘要:

    目的:本研究通过《灵枢.九针十二原》描述的首类针刺补泻法对小型猪皮下组织液压(IFP)的作用,探讨其调节组织液的生物力学机制。方法:分析该法的现代语义,在9只健康实验用小型猪腹部随机取点行补法(提法、按法)和泻法(摇大针孔),在正常状态、抽取组织液的低组织液量状态(LVS)、和注入生理盐水的高组织液量状态(HVS)下操作,对照组仅留针无手法,监测针刺前后的IFP。结果:正常状态下,提/按法可极显著升高IFP;泻法可极显著降低IFP,针后5min泻法组IFP降低较快。LVS下,提/按法均能升高IFP,针后10min二者IFP下降较慢。HVS下,泻法可极显著降低IFP,针后5min与对照组变化趋势不同。结论:该补泻法可升高或降低IFP,证明其对IFP有相反方向的调节作用。

    Abstract:

    Objective In this study, the effect of a reinforcing and reducing method of acupuncture described in nine needles and twelve yuan of lingshu, that effected on the interstitial fluid pressure in subcutaneous connective tissue of mini pigs was observed, and the biomechanical mechanism of regulating the tissue fluid was discussed. The first of the reinforcing and reducing method of acupuncture is recorded at the beginning of the Miraculous Pivot. In this study, we observed the effect of this method on the IFP of the skin in minipigs, which discussed the objective law and mechanism of regulating the interstitial fluid. Methods The manipulation technique was explained in contemporary Chinese language. Then, 9 healthy minipigs were randomly selected for reinforcing (press or bring up) and reducing (wave a big pinhole) and testing on the soft skin tissue of abdomen. In the normal state (divided into BT group, BA group, BD group, and XF group, XD group), the low fluid volume state (LVS) formed by extracting of interstitial fluid (divided into DT group, DA group and DC group) and the high fluid volume state (HVS) by injecting saline solution (divided into GX group and GD group), the IFP was testing before and after acupuncture. Results In the normal state, bringing up and pressing the needle could increase IFP , and reducing method could decrease it(P<0.01) which could increase the rate of descent in 5 min after needling. In LVS, it increases in DT group and DA group(P>0.05). However, in 10 min after needling, the rate of descend of IFP in these two groups are slower. In HVS, that of GX group is significantly lower than GD group (P<0.01), and the changing trend in 5 min after needling is different. Conclusion This method, as recorded in nine needles and twelve yuan of lingshu, can increase or decrease IFP, which proves that the acupuncture method can regulate the interstitial fluid pressure in the opposite direction.

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  • 收稿日期:2019-03-25
  • 最后修改日期:2019-05-13
  • 录用日期:2019-05-30
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