With the development of third-generation high-throughput sequencing technology and tissue engineering, many long-chain non-coding RNAs (LncRNAs) have been found to play an important role in the osteogenic differentiation of mesenchymal stem cells. LncRNAs, which are involved in the regulation of mechanical regulation, further regulate bone-related cell functions and play a regulatory role at multiple levels, including transcription, post-transcriptional and epigenetic. LncRNAs may be involved in the osteogenic differentiation and bone remodeling of MSCs and the regulation of bone-related cell functions as a mechanical response molecule, and participate in the pathological process of skeletal diseases.