载药敷料体外细胞毒性及释药性评价
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天津市自然科学基金重点项目(09JCZDJC18800);军事医学科学院院创新基金重点项目(2008ZD009)


Evaluation of in vitro cytotoxicity and drug release property on pharmaceutical dressing
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    摘要:

    目的 评价载药敷料体外细胞毒性和释药性。方法 按国标GB/T14233.2-2005,规定的MTT法操作,通过光学倒置显微镜观察L929细胞形态和增殖状况定性分析载药敷料体外细胞毒性,通过吸光度(OD)值计算细胞相对增殖率定量分析载药敷料体外细胞毒性等级;以磷酸盐缓冲液(PBS7.4)为溶出介质,32℃±0.5℃模拟人体表皮温度,于1/6 h、1/2 h、1h、3h、16h、24h、36h、48h不同时间点取样,采用紫外分光光度法测定载药敷料体外累积释放性能。结果 该载药敷料浸提原液中的细胞形态正常,贴壁生长良好,细胞平均相对增殖率(RGR)为91.25%,毒性为1级。载药敷料的释药动力学符合Higuchi方程:拟合方程为Mt/M∞=0.3271t0.239。结论 该载药敷料细胞相容性良好,并具有一定的缓释性能。

    Abstract:

    Objective To evaluate the cellular toxic and release of pharmaceutical dressing. Methods Following the State standard GB/T14233.2-2005, the L929 cellular morphology was observed by inverted microscopy after 72h and proliferation of the cells was examined using mitochondrial function (MTT) assay. Relative growth rate (RGR) was calculated and cytotoxicity grade was evaluated. With PBS7.4 as dissolution media, and (32±0.5)℃ as dissolution temperature, the release rate was determined with UV method with the determination wavelength of 288nm and the dissolved liquid in1/6, 1/2, 1, 3, 16, 24, 36 and48h. Results The average cell RGR of the pharmaceutical dressing was 91.25% and reached 1 grade. L929 cellular morphology was normal. Pharmaceutical dressing release accord to Higuchi equation,and the simulated equation is Mt/M∞=0.3271t0.239. Conclusion Biologic compatibility of the pharmaceutical dressing is good, and the release of levofloxacin from the pharmaceutical dressing is sustained in vitro.

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黄姝杰,关静,李志宏,张西正,武继民.载药敷料体外细胞毒性及释药性评价[J].医用生物力学,2010,25(1):32-35

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